Main conclusion Nicotiana benthamiana plants transiently transformed with the C4 vector are capable to produce curcumin, demethoxycurcumin and bisdemethoxycurcumin in reasonable yields. Abstract Curcuminoids are valuable bioactive compounds whose heterologous production remains limited by precursor availability, cytotoxic intermediates and imbalanced metabolite ratios in microbial systems. In this study, we employed Nicotiana...