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This study focuses on enhancing L-Dopa synthesis by producing tyrosine hydroxylase from Bacillus subtilis SDSC-Env-i6 and its conjugation with aluminium oxide (Al₂O₃) nanoparticles. The free enzyme exhibited moderate activity, which was significantly improved through nanoparticle conjugation. Structural characterisation confirmed successful binding, with Al₂O₃ nanoparticles showing a crystalline nature and sizes ranging from 1...
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