The CRISPR-Cas13 system has emerged as a powerful platform for programmable RNA targeting, offering efficient and sequence-specific silencing of coding and non-coding transcripts. The RNA-targeting capabilities of CRISPR-Cas13 have been harnessed to silence transcripts harbouring pathogenic mutations and combat infectious diseases. However, the molecular basis of on-target and collateral activity are not completely understood,...

Avian influenza virus (AIV) remains a persistent threat to global poultry production and public health, owing to its ability to infect a wide range of avian and mammalian species. The global resurgence of H5N1 and the limitations associated with control measures in intensive poultry production have highlighted the need for a paradigm shift in disease management strategies. In this context, the development of AIV resilient poul...