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Springer Nature1
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Marine Biotechnology1
Publication Year
20241
Axmee Regmi results 1 · Newest (Page 1/1, per page 25)
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Exploring the Use of Alternative Promoters for Enhanced Transgene and sgRNA Expression in Atlantic Salmon CellsNARA Subscribed
Marine Biotechnology · 2024 · Vol. 26 · Issue 6 · Springer
Mohammad Ali Noman Reza; Thomas Nelson Harvey; Axmee Regmi; Jacob Seilø Torgersen; Guro Katrine Sandvik
This study facilitates design of expression vectors and lentivirus tools for gene editing of Atlantic salmon. We have characterized widely used heterologous promoters and novel endogenous promoters in Atlantic salmon cells. We used qPCR to evaluate the activity of several U6 promoters for sgRNA expression, including human U6 (hU6), tilapia U6 (tU6), mouse U6 (mU6), zebrafish U6 (zU6), Atlantic salmon U6 (sU6), medaka U6 (medU6...
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