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Genetic ancestry proportion influences risk of adverse events from tuberculosis treatment in BrazilNARA Subscribed
Tuberculosis (TB) treatment is highly effective, but response to therapy varies by geography and population subgroups. We assessed differences in TB treatment response in a representative and heterogeneous Brazilian population. We estimated genetic ancestry according to major genetic ancestry groups (African, European, and Amerindian) in the Regional Prospective Observational Research in Tuberculosis (RePORT)-Brazil cohort usi...
The in vitro alkaline comet assay with liver models as a complementary tool for genotoxicity assessment of N-nitrosaminesNARA Subscribed
N -nitrosamine (NA) impurities in pharmaceuticals represent “cohort of concern” compounds under ICH M7(R2), due to their mutagenic/carcinogenic potential, involving cytochrome P450 (CYP)-mediated metabolic activation. Increasing interest in mammalian cell-based genotoxicity/mutagenicity assays prompted our assessment of the in vitro alkaline comet assay regarding its predictive power for NAs. Here, precision-cut liver slices (...
Soil water availability modulates the response of grapevine leaf gas exchange and PSII traits to a simulated heat waveNARA Subscribed
Background and aims A better understanding of plant carbon assimilation, water status and photosystem performance responses to combined heat and drought stress would help to optimize grapevine management under such limiting conditions. Methods Gas exchange and chlorophyll fluorescence parameters were measured in potted grapevines, cv Sauvignon Blanc, before, during and after simulated six-day heat (T max = 40 °C) wave using he...
Fibulorhizoctonia psychrophila, causal agent of lenticel spot in pome fruit, rarely detected in Dutch pear and apple orchardsNARA Subscribed
Fibulorhizoctonia psychrophila causes lenticel spot in stored pome fruit. A Dutch survey revealed that damage can be observed regularly in individual lots at low incidence to occasionally incidences above 25% after storage for five months or longer under specific controlled atmosphere storage conditions. The source of inoculum of the psychrophilic pathogen is not known. A species-specific TaqMan PCR assay was developed to quan...
The quorum‐sensing systems of Vibrio campbellii DS40M4 and BB120 are genetically and functionally distinctNARA Subscribed
Summary Vibrio campbellii BB120 (previously classified as Vibrio harveyi ) is a fundamental model strain for studying quorum sensing in vibrios. A phylogenetic evaluation of sequenced Vibrio strains in Genbank revealed that BB120 is closely related to the environmental isolate V. campbellii DS40M4. We exploited DS40M4's competence for exogenous DNA uptake to rapidly generate greater than 30 isogenic strains with deletions of g...
The rapidly advancing field of metabolomics encompasses a diverse suite of powerful analytical and bioinformatic tools that can help to reveal the diversity and activity of chemical compounds in individual organisms, species interactions, and entire ecosystems. In this perspective we use examples from studies of coral reefs to illustrate ways in which metabolomics has been and can be applied to understand coastal ecosystems. E...
Key message Bryophytes as models to study the male germ line: loss-of-function mutants of epigenetic regulators HAG1 and SWI3a/b demonstrate conserved function in sexual reproduction. Abstract With the water-to-land transition, land plants evolved a peculiar haplodiplontic life cycle in which both the haploid gametophyte and the diploid sporophyte are multicellular. The switch between these phases was coined alternation of gen...
Three-Dimensional Molecular Cartography of the Caribbean Reef-Building Coral Orbicella faveolataOA Marine
All organisms host a diversity of associated viruses, bacteria, and protists, collectively defined as the holobiont. While scientific advancements have enhanced the understanding of the functional roles played by various components of the holobiont, there is a growing need to integrate multiple types of molecular data into spatially and temporally resolved frameworks. To that end, we mapped 16S and 18S rDNA metabarcoding, meta...
Combinatorial optimization applied to VLBI schedulingNARA Subscribed
Due to the advent of powerful solvers, today linear programming has seen many applications in production and routing. In this publication, we present mixed-integer linear programming as applied to scheduling geodetic very-long-baseline interferometry (VLBI) observations. The approach uses combinatorial optimization and formulates the scheduling task as a mixed-integer linear program. Within this new method, the schedule is con...