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Journal of Fish Biology · 2013 · Vol. 82 · Issue 3 · Wiley
Repeated non‐invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single‐cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide ( H 2 O 2 , 0–200 µM ), and in vitro exposure of sperm to 200 µM H 2 O 2 produced 88·7 ± 3·9% tail DNA compared to unexposed co...