This study evaluated the effects of different frozen concentrated microalgal feeds and their mixtures on the growth, digestive enzyme activity, biochemical composition, and metabolomic profiles of adult Manila clams, Ruditapes philippinarum, aiming to optimize feeding strategies for clam aquaculture. Clams were fed four diets: single species of Chlorella pyrenoidosa, Isochrysis galbana 3011, or Chaetoceros muelleri, and a mixe...