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Aim To delineate native freshwater fish biogeographic regions in the Philippine archipelago and identify the contemporary and historical factors that structure freshwater fish diversity, endemism and species turnover. Location The Philippine archipelago. Taxon Native freshwater fishes. Methods We compiled an archipelago‐wide dataset of native freshwater fish occurrences and integrated these with environmental variables and his...
Microbial communities in sandy beaches from the three domains of life differ by microhabitat and intertidal locationNARA Subscribed
The microbial communities of sandy beaches are poorly described despite the biogeochemical importance and ubiquity of these ecosystems. Using metabarcoding of the 16S and 18S rRNA genes, we investigated the diversity, microhabitats (with or between sand grains) and intertidal distributions of microorganisms (including meiofauna) from pristine sandy beaches in British Columbia, Canada, and hypothesized that abiotic variations d...
Validation of a universal set of primers to study animal‐associated microeukaryotic communitiesNARA Subscribed
Summary The application of metabarcoding to study animal‐associated microeukaryotes has been restricted because the universal barcode used to study microeukaryotic ecology and distribution in the environment, the Small Subunit of the Ribosomal RNA gene (18S rRNA), is also present in the host. As a result, when host‐associated microbial eukaryotes are analysed by metabarcoding, the reads tend to be dominated by host sequences....
Although free‐living protists play essential roles in aquatic and soil ecology, little is known about their diversity and phylogeography, especially in terrestrial ecosystems. We used mitochondrial cytochrome c oxidase subunit 1 (COI) gene sequences to investigate the genetic diversity and phylogeography of the testate amoeba morphospecies Hyalosphenia papilio in 42 Sphagnum (moss) ‐ dominated peatlands in North America, Europ...