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Formalin‐inactivated vaccines are widely employed as a primary preventive strategy against red sea bream iridoviral disease (RSIVD), which poses a substantial economic threat to the aquaculture of rock bream ( Oplegnathus fasciatus ). However, conventional quantitative PCR (qPCR) cannot differentiate infectious virions from noninfectious vaccine residues or viral debris, limiting accurate assessment of vaccine efficacy. Theref...
Development and Validation of a Viability RT ‐ qPCR Assay for Detecting Infectious Spring Viraemia of Carp Virus ( SVCV )NARA Subscribed
Spring viremia of carp virus (SVCV) is a contagious pathogen associated with significant mortality and economic losses in freshwater aquaculture. Although reverse transcription quantitative PCR (RT‐qPCR) enables rapid detection, it does not distinguish between infectious and non‐infectious viral particles, which may lead to overestimation of infection risk. In this study, a viability RT‐qPCR (vqPCR) assay targeting the glycopr...
Foliar application of inorganic nutrients and organic acids can affect the metabolic profile of soybean leaves and enhance soybean productivity. In this study, we investigated whether foliar application of 12 inorganic nutrients [N, P, K (10 g L −1 each); Ca, Mg, B, Zn, Fe, Cu, Mo, Si (200 g L −1 each); Se (20 g L −1 )] and five organic acids [acetic, ascorbic, citric, formic, and malic acids (200 mM each)] could alter seconda...
Physiological Effects of Suspended Solids on Venerupis philippinarum and Argopecten irradiansOA Marine
Suspended solids are small particles transported in the water column, which can damage marine ecosystems and impair the health of aquatic organisms. This study evaluated the physiological responses of clams (Venerupis philippinarum) and Atlantic Bay scallops (Argopecten irradians) to suspended solid exposure. Four concentrations (100–1000 mg/L) were tested, with a control group maintained at 0 mg/L. At each time point (1, 2, 4...
The objective of this study was to investigate the pharmacokinetic (PK) properties of oxolinic acid (OA) in black rockfish ( Sebastes schlegelii ) following a single oral administration of 30 mg/kg at 13°C and 22°C. Serum and tissue (muscle, liver and kidney) samples were collected at pre‐determined time points and analysed using high‐performance liquid chromatography coupled with tandem mass spectrometry (HPLC‐MS/MS). The res...