Background While porcine Neonatal Pancreatic Cell Clusters (NPCCs) were potential alternative source of islets, their immaturity and heterogeneity make them inefficiently ameliorate hyperglycemia in rodents. Our previous work showed that NPCCs regress to a transitional-like state immediately after isolation and undergo dynamic transcriptional changes during short-term in vitro culture. The present study aimed to further deline...

Introduction Offshore wind farms (OWFs) are vital for sustainable energy. The underwater foundations of those wind turbines provide a complex structure that creates new habitats and attracts fishes. However, whether OWFs had the same ecological function as purpose-built artificial reef (AR) and how they alter the original fish community remain unclear. Methods To evaluate the spatial and temporal variations in fish assemblages...

To effectively conserve coral reef ecosystems, it is essential to monitor changes in fish fauna using minimally invasive methods. In this study, we conducted a fish survey in the Dongsha Atoll using eDNA metabarcoding. Analyzing 37 sequencing units from 26 water samples collected across 12 sites from May 2017 to September 2018, we identified 421 species from 79 families. Among these, 192 species had not been recorded in previo...