Purpose Tacrolimus (TAC) is known for its high pharmacokinetic variability which cannot be fully explained by pharmacogenomic (PGx) and clinical variables. We identified gut microbiome associated with TAC pharmacokinetic variability in allogeneic hematopoietic cell transplant (HCT) recipients. Methods In this observational study, metagenomic shotgun sequencing was used to analyze stool microbiome collected within ± 10 days fro...

Summary In this minireview, we expand upon traditional microbial source tracking (MST) methods by discussing two recently developed, next‐generation‐sequencing (NGS)‐based MST approaches to identify sources of fecal pollution in recreational waters. One method defines operational taxonomic units (OTUs) that are specific to a fecal source, e.g., humans and animals or shared among multiple fecal sources to determine the magnitud...

Summary V ibrio vulnificus is a potentially lethal human pathogen that occurs naturally in estuarine waters and shellfish. V ibrio vulnificus was quantified in water and oysters from F lorida's G ulf C oast by plating on mCPC agar, enrichment and plating, and quantitative PCR ( qPCR ). V ibrio vulnificus was detected in 19%, 29%, and 97% of samples respectively by direct plating, qPCR , and enrichment. Only 8% of typical colon...

Summary Chemical profiles of the Black Sea suboxic zone show a distribution of nitrogen species which is traditionally associated with denitrification, i.e. a secondary nitrite maximum associated with nitrate depletion and a N 2 gas peak. To better understand the distribution and diversity of the denitrifier community in the Black Sea suboxic zone, we combined a cultivation approach with cloning and sequencing of PCR‐amplified...