In this study, a new cell line named yellowfin sea bream muscle (YSBM) was successfully established by using muscle tissue from yellowfin seabream, Acanthopagrus latus , a widespread saline fish cultivated in China. The YSBM cell line was subcultured more than 100 passages and appeared to be suitable for researching gene functions in vitro, as it was able to be transfected successfully with pEGFP‐N3 plasmid. Additionally, YSBM...