Boletellu s and Aureoboletus are among the most prominent bolete genera in the family Boletaceae . The diversity of these taxa in the Caribbean is high, but their species delimitation is still critical. An inclusive phylogenetic reconstruction of these genera is achieved by sequencing five loci: nuclear ribosomal DNA regions (ITS, LSU) and protein coding genes ( TEF 1-α, RPB 1, and RPB 2). Three target species from the Greater...

Only two Coccoloba -associated xerocomoid boletes with smooth basidiospores are currently known from the Dominican Republic, namely Boletus ruborculus and Xerocomus coccolobae . A multilocus phylogenetic analysis of four gene markers (ITS, LSU, RPB2 , TEF1 ) reveals that B . ruborculus forms an autonomous clade in the Boletaceae corresponding to a novel genus, which is introduced here as Tropicoboletus gen. nov., whereas X. co...