Biofilms are essential for plant‐associated bacteria to colonize their host. In this work, we analysed the interaction of Azospirillum baldaniorum Sp245 and Pseudomonas fluorescens A506 in mixed macrocolony biofilms. We identified certain culture conditions where A. baldaniorum Sp245 exploits P. fluorescens A506 to boost its growth. Azospirillum growth increased proportionally to the initial number of pseudomonads building the...

Summary Pseudomonas donghuensis strain SVBP6, an isolate from an agricultural plot in Argentina, displays a broad‐spectrum and diffusible antifungal activity, which requires a functional gacS gene but could not be ascribed yet to known secondary metabolites typical of Pseudomonas biocontrol species. Here, we report that Tn 5 mutagenesis allowed the identification of a gene cluster involved in both the fungal antagonism and the...

Summary A main goal of biological nitrogen fixation research has been to expand the nitrogen‐fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen‐fixing rhizobacterium Pseudomonas protegens Pf‐5 X940 as a chassis to engineer the transfer of nitrogen fixed by BNF to maize and wheat under non‐gnotobiotic conditions. Inoculation of maize and wheat with Pf‐5 X940 largely improved nit...