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In Vitro Cellular & Developmental Biology - Plant · 2023 · Vol. 59 · Issue 6 · Springer
A reliable and simple Agrobacterium -mediated transformation system for the unicellular green algae model organism Chlamydomonas reinhardtii has been developed. The protocol has been successfully employed with both neomycin phosphotransferase II ( nptII ) and the phleomycin resistance ( bleI ) genes coupled with the selective agents paromomycin and zeocin, respectively. A set of binary vectors were assembled that carry the sel...