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Pre‐existing phytoplankton biomass concentrations shape coastal plankton response to fire‐generated ash leachateNARA Subscribed
Climate‐driven warming is projected to intensify wildfires, increasing their frequency and severity globally. Wildfires are an increasingly significant source of atmospheric deposition, delivering nutrients, organic matter, and trace metals to coastal and open ocean waters. These inputs have the potential to fertilize or inhibit microbial growth, yet their ecological impacts remain poorly understood. This study examines how as...
Microbial Community Metabolism of Coral Reef Exometabolomes Broadens the Chemodiversity of Labile Dissolved Organic MatterNARA Subscribed
Dissolved organic matter (DOM) comprises diverse compounds with variable bioavailability across aquatic ecosystems. The sources and quantities of DOM can influence microbial growth and community structure with effects on biogeochemical processes. To investigate the chemodiversity of labile DOM in tropical reef waters, we tracked microbial utilisation of over 3000 untargeted mass spectrometry ion features exuded from two coral...
The mutant mouse resource and research center (MMRRC) consortium: the US-based public mouse repository systemNARA Subscribed
Now in its 25th year, the Mutant Mouse Resource and Research Center (MMRRC) consortium continues to serve the United States and international biomedical scientific community as a public repository and distribution archive of laboratory mouse models of human disease for research. Supported by the National Institutes of Health (NIH), the MMRRC consists of 4 regionally distributed and dedicated vivaria, offices, and specialized l...
Microbial respiration is a critical component of the marine carbon cycle, determining the proportion of fixed carbon that is subject to remineralization as opposed to being available for export to the ocean depths. Despite its importance, methodological constraints have led to an inadequate understanding of this process, especially in low-activity oligotrophic and mesopelagic regions. Here, we quantify respiration rates as low...
Seasonal and daily patterns in known dissolved metabolites in the northwestern Sargasso SeaNARA Subscribed
Organic carbon in seawater plays a significant role in the global carbon cycle. The concentration and composition of dissolved organic carbon reflect the activity of the biological community and chemical reactions that occur in seawater. From 2016 to 2019, we repeatedly sampled the oligotrophic northwest Sargasso Sea in the vicinity of the Bermuda Atlantic Time‐series Study site (BATS) to quantitatively follow known compounds...
Integrating phytoplankton pigment and DNA meta‐barcoding observations to determine phytoplankton composition in the coastal oceanNARA Subscribed
Quantifying phytoplankton composition is critical to predicting marine ecosystem structure and function. DNA meta‐barcoding and high‐performance liquid chromatography (HPLC) pigment analysis are two widely used methods for assessing phytoplankton composition; however, comparing their performance has been done only rarely. Here, we integrate DNA meta‐barcoding and HPLC pigment observations to determine eukaryotic phytoplankton...
Carbon content, carbon fixation yield and dissolved organic carbon release from diverse marine nitrifiersNARA Subscribed
Nitrifying microorganisms, including ammonia‐oxidizing archaea, ammonia‐oxidizing bacteria, and nitrite‐oxidizing bacteria, are the most abundant chemoautotrophs in the ocean and play an important role in the global carbon cycle by fixing dissolved inorganic carbon (DIC) into biomass. The release of organic compounds by these microbes is not well quantified, but may represent an as‐yet unaccounted source of dissolved organic c...
This document describes best practices for analysis of dissolved organic matter (dissolved organic carbon and total dissolved nitrogen) in seawater samples. Included are SOPs for sample collection and storage, details for laboratory analysis using high temperature combustion analysis on Shimadzu TOC analyzers, and suggestions for best practices in quality control and quality assurance. Although written specifically for GO-SHIP...
Bacterioplankton communities reveal horizontal and vertical influence of an Island Mass EffectNARA Subscribed
Summary Coral reefs are highly productive ecosystems with distinct biogeochemistry and biology nestled within unproductive oligotrophic gyres. Coral reef islands have often been associated with a nearshore enhancement in phytoplankton, a phenomenon known as the Island Mass Effect (IME). Despite being documented more than 60 years ago, much remains unknown about the extent and drivers of IMEs. Here we utilized 16S rRNA gene met...
Subcortical brain structure in children with developmental coordination disorder: A T1-weighted volumetric studyNARA Subscribed
Developmental coordination disorder (DCD) is a neurodevelopmental disorder occurring in 5–6% of school-aged children. Converging evidence suggests that dysfunction within cortico-striatal and cortico-cerebellar networks may contribute to motor deficits in DCD, yet limited research has examined the brain morphology of these regions. Using T1-weighted magnetic resonance imaging the current study investigated cortical and subcort...
Ocean warming is causing global coral bleaching events to increase in frequency, resulting in widespread coral mortality and disrupting the function of coral reef ecosystems. However, even during mass bleaching events, many corals resist bleaching despite exposure to abnormally high temperatures. While the physiological effects of bleaching have been well documented, the consequences of heat stress for bleaching‐resistant indi...
Planktonic communities constitute the basis of life in marine environments and have profound impacts in geochemical cycles. In the North Atlantic, seasonality drives annual transitions in the ecology of the water column. Phytoplankton bloom annually in spring as a result of these transitions, creating one of the major biological pulses in productivity on earth. The timing and geographical distribution of the spring bloom as we...
To date, the relative contribution of primary marine organic matter to the subset of atmospheric particles that nucleate cloud droplets is highly uncertain. Here, cloud condensation nuclei (CCN) measurements were conducted on aerosolized sea surface microlayer (SML) samples collected from the North Atlantic Ocean during the NASA North Atlantic Aerosols and Marine Ecosystems Study (NAAMES), κ values were predicted for three rep...
The bioavailability of organic matter (OM) to marine heterotrophic bacterioplankton is determined by both the chemical composition of OM and the microbial community composition. In the current study, changes in OM bioavailability were identified at Ocean Station Papa as part of the 2018 Export Processes in the Ocean from Remote Sensing (EXPORTS) field study. Removal rates of carbon (C) in controlled experiments were significan...
Particulate organic matter (POM) (fecal pellets) from zooplankton has been demonstrated to be an important nutrient source for the pelagic prokaryotic community. Significantly less is known about the chemical composition of the dissolved organic matter (DOM) produced by these eukaryotes and its influence on pelagic ecosystem structure. Zooplankton migrators, which daily transport surface-derived compounds to depth, may act as...
Marine dissolved organic matter (DOM) varies in its recalcitrance to rapid microbial degradation. DOM of varying recalcitrance can be exported from the ocean surface to depth by subduction or convective mixing and oxidized over months to decades in deeper seawater. Carboxyl‐rich alicyclic molecules (CRAM) are characterized as a major component of recalcitrant DOM throughout the oceanic water column. The oxidation of CRAM‐like...
Mesoscale eddies, prominent physical processes in the stratified ocean gyres, affect community composition and metabolic rates of both phytoplankton and heterotrophic bacterioplankton (free‐living bacterial and archaeal) communities. We hypothesized that in situ differences in organic matter production would predispose bacterioplankton communities from cyclonic vs. anticyclonic eddies toward metabolic capabilities better suite...