Atmospheric carbon dioxide (CO 2 ) is fixed by mangrove vegetation and stored in its biomass and sediments. Part of the sediment carbon can be exported to coastal waters via tidally driven pore‐water exchange. Here, we quantify pore water‐derived dissolved CO 2 export using in situ, high‐resolution observations of 222 Rn and CO 2 over a spring‐neap tidal cycle in a mangrove‐fringed estuary (Coffs Creek, Australia). 222 Rn‐deri...

Flavobacterium psychrophilum is the aetiologic agent of bacterial coldwater disease and rainbow trout fry syndrome. In this study, we compared a wild‐type strain (CSF 259‐93) with a rifampicin‐resistant strain and virulence‐attenuated strain of F. psychrophilum (CSF 259‐93B.17). The attenuated strain harboured a mutation in the rpoB gene consistent with resistance to rifampicin. Two‐dimensional polyacrylamide gel electrophores...

It is hypothesized that the frequency of bacterial coldwater disease outbreaks can be reduced through the detection of the aetiologic agent, Flavobacterium psychrophilum , in broodstock followed by culling of eggs from heavily infected broodstock. Before a culling programme can be instituted, however, it is necessary to determine the sensitivity and specificity of existing assays for the detection of F. psychrophilum . In this...

Strawberry disease (SD) is an inflammatory skin disorder in rainbow trout, Oncorhynchus mykiss (Walbaum). The aetiology of SD is unknown although the 16S rDNA sequence of a Rickettsia ‐like organism (RLO) has been associated with SD lesions using a nested PCR assay. In this study, we developed a Taqman quantitative PCR assay (qPCR) that targeted the RLO 16S rDNA sequence to examine the distribution of RLO relative to lesion st...

Red‐mark syndrome (RMS), a disease seen mostly in rainbow trout, Oncorhynchus mykiss , is of unknown aetiology. The research presented here indicates the presence of an intracellular bacterium in RMS‐affected fish. A positive reaction was observed using immunohistochemistry (IHC) with skin lesions, liver, kidney and spleen of affected fish sampled from several locations within the United Kingdom using two different polyclonal...

Flavobacterium psychrophilum is the aetiological agent of rainbow trout fry syndrome and bacterial cold water disease. This study examined the genetic diversity of F. psychrophilum isolates retrieved from multiple epizootics at rainbow trout, Oncorhynchus mykiss , rearing facilities and from spawning coho salmon, O. kisutch . A total of 139 isolates were confirmed as F. psychrophilum by PCR assay and were further typed using p...