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Journal of Fish Biology · 2010 · Vol. 77 · Issue 5 · Wiley
Embryonic stem (ES)‐like cells were derived from mid‐blastula stage embryos of a freshwater fish, catla Catla catla , under feeder‐free condition and designated as CCES cells. The conditioned media was optimized with 10% foetal bovine serum (FBS), fish embryo extract (FEE) having 100 µg ml −1 protein concentration, 15 ng ml −1 basic fibroblast growth factor (bFGF) and basic media containing Leibovitz‐15, DMEM with 4·5 g l −1 g...