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Correlation between DNA methylation and transcriptomic changes in golden Asian sea bass ( Lates calcarifer ): An integrated approachNARA Subscribed
Lates calcarifer , commonly known as barramundi perch, Asian sea bass or barramundi, is a significant aquaculture species across the Indo‐Pacific. Barramundi typically exhibit a silver to bronze wild‐type (WT) skin colour, with occasional golden (xanthic) variants of commercial interest. Although previous studies have identified gene expression differences between golden and WT variants, the genetic mechanism driving golden co...
Optimising commercial traits through gene editing in aquaculture: Strategies for accelerating genetic improvementNARA Subscribed
Aquaculture is one of the fastest‐growing food production sectors. As the global human population continues to increase and further pressure is added to the prospects of achieving global food security, aquaculture is expected to play an integral role in meeting future nutrition demands. With advances in genetic technologies over recent years, much progress has been made within the realm of selective breeding. Despite success,...
Towards a ‘systems’ approach for viral challenge experiments in shrimp: Reporting guidelines for publicationNARA Subscribed
The success of contemporary disease management strategies in shrimp aquaculture, such as the ‘systems’ approach, is predicated on robust knowledge of the conditions and interactions between the host, pathogen and environment that promote disease. Pathogen challenge experiments (PCEs) are a power tool for investigating these conditions and interactions. However, absence of accurately reported experimental detail in published PC...
Multi-modal MRI of hippocampal morphometry and connectivity after pediatric severe TBINARA Subscribed
This investigation explores memory performance using the California Verbal Learning Test in relation to morphometric and connectivity measures of the memory network in severe traumatic brain injury. Twenty-two adolescents with severe traumatic brain injury were recruited for multimodal MRI scanning 1–2 years post-injury at 13 participating sites. Analyses included hippocampal volume derived from anatomical T1-weighted imaging,...
Conservation of Dry Forest Old Growth in Eastern OregonNARA Subscribed
Conservation of old-growth forests has become an increasingly important objective of Forest Service managers over the last three decades. The US Forest Service recently made changes to policies that prohibit cutting of live trees >53 cm (the “21-inch rule”). We review the disturbance ecology of dry and mesic old-growth forests of Oregon and contrast conservation policies for these two forest types. We describe the development...
Lengthening of maize maturity time is not a widespread climate change adaptation strategy in the US MidwestNARA Subscribed
Increasing temperatures in the US Midwest are projected to reduce maize yields because warmer temperatures hasten reproductive development and, as a result, shorten the grain fill period. However, there is widespread expectation that farmers will mitigate projected yield losses by planting longer season hybrids that lengthen the grain fill period. Here, we ask: (a) how current hybrid maturity length relates to thermal availabi...
Current status of parasitic ciliates Chilodonella spp. (Phyllopharyngea: Chilodonellidae) in freshwater fish aquacultureNARA Subscribed
Freshwater fish farming contributes to more than two‐thirds of global aquaculture production. Parasitic ciliates are one of the largest causes of production loss in freshwater farmed fishes, with species from the genus Chilodonella being particularly problematic. While Chilodonella spp. include ‘free‐living’ fauna, some species are involved in mortality events of fish, particularly in high‐density aquaculture. Indeed, chilodon...
The complex relationship between personal sense of connection to animals and self‐reported proenvironmental behaviors by zoo visitorsNARA Subscribed
The global biodiversity crisis requires an engaged citizenry that provides collective support for public policies and recognizes the consequences of personal consumption decisions. Understanding the factors that affect personal engagement in proenvironmental behaviors is essential for the development of actionable conservation solutions. Zoos and aquariums may be some of the only places where many people can explore their rela...
The importance of managing land to optimize carbon sequestration for climate change mitigation is widely recognized, with grasslands being identified as having the potential to sequester additional carbon. However, most soil carbon inventories only consider surface soils, and most large‐scale surveys group ecosystems into broad habitats without considering management intensity. Consequently, little is known about the quantity...
Abiotic drivers and plant traits explain landscape‐scale patterns in soil microbial communitiesNARA Subscribed
The controls on aboveground community composition and diversity have been extensively studied, but our understanding of the drivers of belowground microbial communities is relatively lacking, despite their importance for ecosystem functioning. In this study, we fitted statistical models to explain landscape‐scale variation in soil microbial community composition using data from 180 sites covering a broad range of grassland typ...
Nitrogen regulates the E arth's climate system by constraining the terrestrial sink for atmospheric CO 2 . Proteolytic enzymes are a principal driver of the within‐system cycle of soil nitrogen, yet there is little to no understanding of their response to climate change. Here, we use a single methodology to investigate potential proteolytic enzyme activity in soils from 16 global change experiments. We show that regardless of...
The gene expression response of the catadromous perciform barramundi Lates calcarifer to an acute heat stressNARA Subscribed
The acute heat‐shock response of the tropical estuarine fish species barramundi Lates calcarifer as indicated by the expression of genes within stress ( hsp 90AA , hsp 90AB , hsp 70 and hsc 70 ), metabolic ( cisy , cco II and ldh ) and growth ( igf1 and mstn 1 ) related pathways was examined following an increase in water temperature from 28 to 36° C over 30 min. Lates calcarifer were maintained at the acute stress temperature...
A robust flow‐cytometric protocol for assessing growth rate of hatchery‐reared barramundi Lates calcarifer larvaeNARA Subscribed
In this study, a flow‐cytometric cell cycle analysis method to assess instantaneous growth rate of whole larvae of the Australian barramundi Lates calcarifer was developed and validated. High‐resolution DNA measurements of either fresh, frozen or RNA later ‐preserved larvae (gap0–gap1, G 0 –G 1 , coefficient of variation ( c.v. ) 0 –G 1 , S (DNA synthesis) and G 2 –M (Gap2–Mitosis). This technique can be also used for individu...