Summary Pseudomonas virus vB_PaeM_PA5oct is proposed as a model jumbo bacteriophage to investigate phage‐bacteria interactions and is a candidate for phage therapy applications. Combining hybrid sequencing, RNA‐Seq and mass spectrometry allowed us to accurately annotate its 286,783 bp genome with 461 coding regions including four non‐coding RNAs (ncRNAs) and 93 virion‐associated proteins. PA5oct relies on the host RNA polymera...