There is emerging interest in tropical C4 forage grasses due to their potential value in changing pasture conditions caused by climate change. In this study, a robust regeneration and transformation system was developed for Rhodes grass ( Chloris gayana ). From elite commercial cultivar ‘Tolgar’ initial screening for embryogenic callus (EC) responses were generated from cut mesocotyl explants, with 20µM of 2,4-D being most eff...

Rhodes grass ( Chloris gayana ) is a warm-season C4 grass currently grown as a forage in some tropical regions, with anticipated future application in areas affected by climate change. However, there are few resources available for this grass, with few genomic resources and only one established transformation system, thus limiting the application of biotechnology methods for its improvement. Protoplast transformation can be us...