Natural oil seeps remain poorly explored in terms of their biodiversity. Most studies on these habitats have focused on prokaryotic communities involved in hydrocarbon metabolism, whereas the microscopic invertebrates inhabiting these environments have received little attention, and existing observations rarely include species-level identifications. Here, we collected samples from a natural oil seep and characterized the meiof...

Species are fundamental units of biodiversity, yet their delimitation remains challenging in many organismal groups. The increasing use of DNA data has revealed widespread cryptic diversity, in which genetically distinct lineages are morphologically indistinguishable. Consequently, many morphology-based biogeographical inferences have likely overestimated species ranges, particularly in microscopic animals traditionally consid...

Aim Freshwater rock pools are ephemeral and fragile habitats that support specialised animal taxa. While distributed worldwide, these habitats are usually neglected and overlooked. We used DNA metabarcoding and metaphylogeographic approaches to study inter and intraspecific tardigrade biodiversity to identify their biogeographic patterns to inform conservation measures. Location Europe. Period Present. Major Taxa Studied Tardi...

Altitudinal gradients offer a unique opportunity to understand the drivers of species richness, as mountain regions cover vast areas and contribute disproportionately to global terrestrial biodiversity. However, such gradients are often studied without considering the role of microhabitats, which introduce fine‐scale heterogeneity within coarse macroenvironmental conditions. This heterogeneity may be especially important for m...

Aim Tardigrades, commonly known as some of the toughest organisms on Earth, like many other microscopic invertebrates, are often overlooked in biodiversity monitoring. Our study aimed to elucidate the impact of environmental disturbance caused by anthropogenic activity on tardigrade diversity and distributions. Location Poland, global occurrence data. Time Period 2023. Major Taxa Studied Tardigrada. Methods We conducted a high...

Previous studies on various organisms have suggested that low doses of ethanol can have stimulatory effects, while higher doses may lead to toxicity, a response known as hormesis. Low ethanol concentrations occur naturally in the environment, particularly in fermenting fruits and flower nectar, where pollinators such as honey bees may encounter it. This study aimed to investigate the potential hormetic effects of low-level eth...

Ramazzottius is a widespread genus of tardigrades with extreme cryptobiotic capabilities. Thanks to its ability to survive desiccation and freezing, this genus is usually recorded from harsh habitats such as exposed mosses and lichens and rock pools. In the last years, research focused on both describing Ramazzottius diversity and revealing the molecular mechanisms behind their cryptobiotic capabilities. Despite the research e...

In this study, we examine the distribution of cryptobiotic abilities, specifically anhydrobiosis and cryobiosis, in tardigrade communities across a habitat gradient from the edge to the centre of a forest bordering a desert. Our study demonstrates no correlation between desiccation and freezing tolerance at the community level. However, communities inhabiting the forest centre show higher cryptobiotic performance, likely due t...

Honey bees ( Apis mellifera ) are one of the most crucial pollinators, providing vital ecosystem services. Their development and functioning depend on essential nutrients and substances found in the environment. While collecting nectar as a vital carbohydrate source, bees routinely encounter low doses of ethanol from yeast fermentation. Yet, the effects of repeated ethanol exposure on bees’ survival and physiology remain poorl...

Microbiota are considered significant in the biology of tardigrades, yet their diversity and distribution remain largely unexplored. This is partly due to the methodological challenges associated with studying the microbiota of small organisms that inhabit microbe‐rich environments. In our study, we characterized the microbiota of 31 species of cultured tardigrades using 16S rRNA amplicon sequencing. We employed various sample...

Recent advances in tardigrade taxonomy have been greatly enhanced by the redescriptions of the type species for particular taxa or species groups. De novo characterisation of these key taxa now allows to describe tardigrade species diversity with improved precision and at higher rate, increasing the momentum towards resolving the taxonomic impediment in these micro-invertebrates. Since its description, Diaforobiotus islandicus...

Understanding the dynamics of speciation and extinction events is one of the most interesting subjects in evolutionary biology that relates to all life forms, even the smallest ones. Tardigrades are microscopic invertebrates that attracted public and scientific attention mostly due to their ability to enter into the diapause stage called cryptobiosis and in such stage resist extremely harsh environmental conditions. However, a...

Rock pools are ephemeral freshwater habitats characterized by their small size, well-defined boundaries, and periodic desiccation, making them ideal model systems to answer numerous ecological questions. Although there are numerous studies on rock pool fauna around the world, tardigrades have only rarely been recorded. We conducted the first tardigrade-focused study on rock pools by quantitatively extracting and classifying th...

Faustina faustina is a conchologically highly diverse forest gastropod with several morphological forms. It is a Carpathian species, but it also occurs in northern isolated localities, where it was probably introduced. We performed the first phylogeographic analysis of 22 populations, based on three molecular markers: COI, ITS-2, and 28S rRNA. Genetic data were complemented by paleo-distribution models of spatial occupancy dur...