Summary Carbofuran‐mineralizing strain Novosphingobium sp. KN65.2 produces the CfdJ enzyme that converts the N ‐methylcarbamate insecticide to carbofuran phenol. Purified CfdJ shows a remarkably low K M towards carbofuran. Together with the carbaryl hydrolase CehA of Rhizobium sp. strain AC100, CfdJ represents a new protein family with several uncharacterized bacterial members outside the proteobacteria. Although both enzymes...