The objective of this study was to perform genomic, phylogenomic, and phenotypic characterization of bacterial isolates from a captive Cacatua alba to investigate their antimicrobial resistance profiles, virulence-associated genes, and genomic relationships with publicly available strains. Bacterial isolates obtained from the lungs and encephalon were identified as Escherichia coli (AV037), Enterobacter hormaechei (AV038), and...

Background Mycobacterium bovis BCG is widely used as a vaccine vector due to its safety, genetic stability, and immunomodulatory properties. However, current expression systems for BCG transformation rely on antibiotic resistance genes, raising biosafety concerns. Therefore, alternative strategies—such as the use of auxotrophic strains—are highly desirable. Our group previously employed BioBrick™ technology to construct the pU...

Background Microsatellite and mitochondrial markers are important tools for investigating natural populations, enabling the assessment of their diversity, genetic structure, kinship, parentage, and reproductive biology. Although the importance of such markers is widely recognized, their use in Neotropical mammals, especially marsupials, is still limited by the scarcity of species-specific loci characterized for extant species....

Uterine leiomyomas (ULMs) are the most common benign gynecological tumors affecting women in reproductive age. Symptoms such as abnormal uterine bleeding, pelvic pain, and infertility may occur, leading to a significant decline in patients’ quality of life. In addition, ULMs represent the second leading indication for gynecological surgery in Brazil, imposing a substantial economic burden on the public healthcare system. Howev...

The insidious onset and progression of sarcopenia make it vital to understand the early skeletal muscle changes and explore therapies to slow its progression. This study explored the gastrocnemius remodeling at an early stage of aging (14 months of age) and the effects of lifelong aerobic exercise. For that, 2-month-old male Wistar rats underwent a 12-month treadmill exercise program. Sedentary age-matched, young sedentary, an...

We evaluated endolithic fungal diversity associated with rocks sampled at the polyextreme Brazilian São Pedro and São Paulo archipelago using a DNA amplicon metagenomics approach. We detected 808,547 fungal DNA reads grouped into 92 amplicon sequence variants (ASVs). The rocks sampled were geologically characterized as mylonitized peridotites, serpentinized peridotites, and carbonate-matrix sedimentary breccias. Ascomycota was...

The quality and leaf yield of Passiflora edulis Sims depend on agricultural management decisions that modulate growth, metabolite accumulation, and antioxidant responses. We compared three commercial agroecosystems in Paty do Alferes, Rio de Janeiro (conventional, organic, and agroforestry), quantifying vegetative growth, leaf biomass, secondary metabolites (total flavonoids and total phenolics), and functional markers (ABTS,...

Yeasts can produce microbial oil from hydrophobic substrates such as glycerol, a byproduct of the biodiesel industry that is often undervalued in the market. Yarrowia lipolytica is capable of degrading glycerol and is considered a model organism for studying lipid accumulation. Different yeast strains and culture conditions can result in varying lipid yields with distinct fatty acid profiles. Thus, in this study, five strains...