Purpose Carbohydrate availability is reduced during prolonged exercise, and this may contribute to the loss of power output at the intensity domain transitions. The aim of this study was to investigate the effects of lowered carbohydrate availability on power output at intensity domain transitions, muscle activation, and gross cycling efficiency in endurance-trained female cyclists. Methods Nine well-trained female cyclists co...

Purpose To assess relationships between heart rate (HR), ventilation (̇ $$\dot V$$ V ˙ E ), and respiratory frequency (F R ) decoupling and durability of the first ventilatory threshold (VT 1 ), and the strength of practical models to predict power output at VT 1 during prolonged exercise. Methods Durability of VT 1 was assessed via measurements of power output at VT 1 before and after ~ 2.5-h of initially moderate-intensity c...

Aim To investigate the impact of prolonged running on speed at the moderate-to-heavy intensity transition, and whether increased eccentric load modifies exercise-induced shifts in the moderate-to-heavy intensity transition. Methods Twelve endurance-trained runners (4 females, 8 males, peak oxygen uptake 51.5 mL kg −1 min −1 in females and 56.1 mL kg −1 min −1 in males) participated. Following trials to assess speed at the firs...

Purpose Power output at the moderate-to-heavy-intensity transition decreases during prolonged exercise, and resilience to this has been termed ‘durability’. The purpose of this study was to assess the relationship between durability and the effect of prolonged exercise on severe-intensity performance, and explore intramuscular correlates of durability. Methods On separate days, 13 well-trained cyclists and triathletes (V̇O 2 p...

Purpose The aims of this study were to: (i) describe the time course of the decrease in power output at the moderate-to-heavy intensity transition during prolonged exercise; (ii) investigate the association between durability of the moderate-to-heavy intensity transition and exercise capacity; and (iii) explore physiological correlates of durability of the moderate-to-heavy intensity transition. Methods Twelve trained cyclists...

There is some evidence for temperature-dependent stimulation of mitochondrial biogenesis; however, the role of elevated muscle temperature during exercise in mitochondrial adaptation to training has not been studied in humans in vivo. The purpose of this study was to determine the role of elevating muscle temperature during exercise in temperate conditions through the application of mild, local heat stress on mitochondrial ada...

Purpose To quantify the effects of prolonged cycling on the rate of ventilation ( $${\dot{\text{V}}}_{{\text{E}}}$$ V ˙ E ), frequency of respiration (F R ), and tidal volume (V T ) associated with the moderate-to-heavy intensity transition. Methods Fourteen endurance-trained cyclists and triathletes (one female) completed an assessment of the moderate-to-heavy intensity transition, determined as the first ventilatory threshol...

Several proteins are implicated in transmembrane fatty acid transport. The purpose of this study was to quantify the variation in fatty acid oxidation rates during exercise explained by skeletal muscle proteins involved in fatty acid transport. Seventeen endurance-trained males underwent a (i) fasted, incremental cycling test to estimate peak whole-body fatty acid oxidation rate (PFO), (ii) resting vastus lateralis microbiopsy...

Purpose Exposure to environmental heat stress increases carbohydrate oxidation and extracellular heat shock protein 70 (HSP70) concentrations during endurance exercise at matched absolute, external work rates. However, a reduction in absolute work rate typically occurs when unacclimated endurance athletes train and/or compete in hot environments. We sought to determine the effect of environmental heat stress on carbohydrate ox...

Purpose To determine the effect of prolonged exercise on moderate-to-heavy intensity transition power output and heart rate. Methods Fourteen endurance-trained cyclists and triathletes took part in the present investigation (13 males, 1 female, V·O 2 peak 59.9 ± 6.8 mL . kg −1. min −1 ). Following a characterisation trial, participants undertook a five-stage incremental step test to determine the power output and heart rate at...

Purpose The three-minute all-out test (3MT), when performed on a laboratory ergometer in a linear mode, can be used to estimate the heavy–severe-intensity transition, or maximum metabolic steady state (MMSS), using the end-test power output. As the 3MT only requires accurate measurement of power output and time, it is possible the 3MT could be used in remote settings using personal equipment without supervision for quantificat...