Summary S phingomonas wittichii RW 1 is a dibenzofuran and dibenzodioxin‐degrading bacterium with potentially interesting properties for bioaugmentation of contaminated sites. In order to understand the capacity of the microorganism to survive in the environment we used a genome‐wide transposon scanning approach. RW 1 transposon libraries were generated with around 22 000 independent insertions. Libraries were grown for an ave...