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Springer Nature1
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Systems Microbiology and Biomanufacturing1
Publication Year
20261
Emily M. Makeev results 1 · Newest (Page 1/1, per page 25)
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Directed evolution of Gram-negative Klebsiella michiganensis M5al for desiccation tolerance and survivability of a spray-drying encapsulation processNARA Subscribed
Systems Microbiology and Biomanufacturing · 2026 · Vol. 6 · Issue 4 · Springer
Benjamin M. Arbaugh; Emily M. Makeev; Matthew L. Cope-Arguello; Tiffany M. Lowe-Power; Tina Jeoh
Spray drying is a scalable method for producing dry microbial inoculants, but Gram-negative bacteria often exhibit poor survival under desiccation stress. We employed a directed-evolution approach to improve the survivability of diazotroph Klebsiella michiganensis M5al during spray-drying encapsulation in cross-linked alginate microcapsules (CLAMs). Wildtype K. michiganensis M5al was serially passaged through the CLAMs spray-d...
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