Horseradish peroxidase (HRP), isolated from horseradish roots, is heavily glycosylated, making it difficult to crystallize. In this work, we produced recombinant HRP in E. coli and obtained an X-ray structure of the ferric enzyme at 1.63 Å resolution. The structure shows that the recombinant HRP contains four disulphide bonds and two calcium ions, which are highly conserved in class III peroxidase enzymes. The heme active site...

The continental shelf of the northwestern Gulf of Mexico harbors extensive reefs and banks that support diverse coral reefs and mesophotic communities. Mesophotic communities range in depth from 40 to 200 m and, in this region, foster some of the densest coral forests [aggregations of mesophotic octocoral, antipatharian, and branching stony coral communities] reported in published literature (10.23 ± 9.31 col/m 2 ). The geolog...

A sequence of major flood events in Britain over the last two decades has prompted questions about the influence of anthropogenic greenhouse gas emissions on flood risk. Such questions are difficult to answer definitively, as a range of other factors are involved, but modelling techniques allow an assessment of how much the chance of occurrence of an event could have been altered by emissions. Here the floods of winter 2013/20...