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Deep learning methods such as Mask R‑CNN enable the precise delineation of single-tree crowns from remote sensing data. However, their segmentation performance still depends on local stand conditions. Using UAV multispectral imagery and lidar canopy height models (CHM), we assessed the influence of tree species composition, stand density, and foliage condition on the robustness of deep-learning-based single-tree segmentation....
Functional characterisation of Target of Rapamycin (TOR) signalling in PhyscomitrellaNARA Subscribed
Key message The evolutionary conserved TOR kinase positively controls growth and development of the moss Physcomitrella, development and function of its chloroplasts, its protein synthesis, and cell-cycle progression. Abstract Target of Rapamycin (TOR) is a conserved protein kinase and an important signalling hub in eukaryotes. The moss Physcomitrella ( Physcomitrium patens ) is a model organism for plant physiology, developme...
Production of human papillomavirus type 16 virus-like particles in Physcomitrella photobioreactorsNARA Subscribed
Key message First production of virus-like particles as a vaccine candidate in a non-vascular plant. Abstract Virus-like particles (VLPs) are self-assembling nanoparticles composed of viral structural proteins which mimic native virions but lack viral DNA and infectivity. VLPs are a resourceful class of biopharmaceuticals applied as subunit vaccines or as delivery vehicles for drugs and nucleic acids. Similar to viruses, VLPs...
Objective To determine if and which types of organisational interventions conducted in small and medium size enterprises (SMEs) in healthcare are effective on mental health and wellbeing. Methods Following PRISMA guidelines, we searched six scientific databases, assessed the methodological quality of eligible studies using QATQS and grouped them into six organisational intervention types for narrative synthesis. Only controlle...
Mathematical and statistical models underlie many of the world's most important fisheries management decisions. Since the 19th century, difficulty calibrating and fitting such models has been used to justify the selection of simple, stationary, single‐species models to aid tactical fisheries management decisions. Whereas these justifications are reasonable, it is imperative that we quantify the value of different levels of mod...
Multifactorial analysis of terminator performance on heterologous gene expression in PhyscomitrellaNARA Subscribed
Key message Characterization of Physcomitrella 3′UTRs across different promoters yields endogenous single and double terminators for usage in molecular pharming. Abstract The production of recombinant proteins for health applications accounts for a large share of the biopharmaceutical market. While many drugs are produced in microbial and mammalian systems, plants gain more attention as expression hosts to produce eukaryotic p...