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Hepatocytes primary culture from the Neotropical fish, trahira Hoplias malabaricus (Bloch)NARA Subscribed
Hepatocytes were isolated and cultured from Hoplias malabaricus using a non‐enzymatic protocol. From 6·9 to 9·4 × 10 7 hepatocytes g −1 of liver were isolated and cell viability was 65–85%. Fish hepatocytes attached well to untreated polystyrene flasks (Corning) as well as on treated surfaces with extracellular matrix proteins, i.e. fibronectin, matrigel or type I collagen coatings. Cells rapidly started migrating and reorgani...
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