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Francesca Mensitieri results 3 · Newest (Page 1/1, per page 25)
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Biotechnology Letters · 2022 · Vol. 44 · Issue 11 · Springer
Objectives The aim of the present work was to identify a time-saving, effective, and low-cost strategy to produce in Escherichia coli a protein chimera representing a fusion anti-SARS-CoV-2 candidate vaccine, consisting of immunogenic and antigenic moieties. Results We overexpressed in E. coli BL21(DE3) a synthetic gene coding for CRM197-RBD, and the target protein was detected in inclusion bodies. CRM197-RBD was solubilized w...