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Transgene-free genome editing in citrus and poplar meristem tissues via biolistic ribonucleoprotein delivery of CRISPR-Cas9NARA Subscribed
Key message Biolistic particle bombardment was used to deliver CRISPR-Cas9 ribonucleoprotein complexes (RNP) into the shoot apical meristem tissue of citrus and axillary meristem tissue of poplar, generating directed mutations in target genes. Abstract The use of meristematic tissues offers a strategic approach to genome editing in woody species, especially those that are recalcitrant to conventional tissue culture, as these r...
Monsoon Climate and Anthropogenic Influences Shape Primate Distributions Across the Southeastern Edge of the Qinghai‐Tibet PlateauNARA Subscribed
The southeastern edge of the Qinghai‐Tibet Plateau (Yunnan, China) exhibits high biodiversity but stark differences in species richness between its western Longitudinal Range Gorge (LRG) and eastern Yunnan Plateau (YP). We collected distribution data for 16 primate species in Yunnan and analyzed palynological records over the past 20 ka from 21 localities to identify the biogeographic, climatic, and anthropogenic factors that...