Humans are exposed to chemicals that leach from plastics, yet many remain data-poor and lack toxicological evaluation. High-throughput transcriptomics (HTTr) provides a scalable way to screen chemicals and generate mechanistic insights relevant to human health risk assessment. We applied HTTr in MCF-7 breast cancer cells to assess chemicals across several concentrations (0.001–50 µM) used in plastics and dyes. Transcriptomic p...

While many jurisdictions have phased out use of bisphenol A (BPA), there is increasing exposure to mixtures of BPA alternatives. Like BPA, some alternatives perturb nuclear hormone receptors and are endocrine disruptors. We used high-throughput transcriptomics (HTTr) to evaluate the potency and modes of action of seven mixtures of BPA alternatives and their 12 individual components in breast cancer cells. Our aim was to explor...