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A number of factors that have been reported to affect transformation/regeneration rate in plants were studied in multiple eucalypt genotypes. These include surfactant, acetosyringone, fipexide, basal media, activated charcoal, ascorbic acid, lipoic acid, proline, cytokinins, sugars, spectinomycin selection, Agrobacterium strains and concentrations, forchlorfenuron, PVP, silver nitrate, and explant heating prior to cocultivatio...