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Environmental Microbiology · 2003 · Vol. 5 · Issue 4 · Wiley
Summary We constructed and characterized a plasmid‐based genetic system that reports the expression of a toluene‐responsive promoter (P tbuA1 ) by effecting an irreversible, heritable change in the biosensor cell. Expression of the reporter gene gfp is strongly repressed in the absence of expression from the P tbuA1 promoter, and high level gfp expression in the original cell and its progeny is mediated by the site‐specific re...