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Gonadotropin‐induced steroidogenic shift towards maturation‐inducing hormone in Japanese yellowtail during final oocyte maturationNARA Subscribed
Intact ovarian follicles, obtained from untreated and human chorionic gonadotropin (HCG) treated Japanese yellowtail Seriola quinqueradiata during different maturational stages, were incubated with radioactive [3H]pregnenolone, [ 3 H]17‐hydroxyprogesterone or [ 14 C] androstenedione and steroid metabolites identified by thin layer chromatography (TLC) followed by recrystallization to constant specific activity. In untreated la...
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