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Summary Denitrifying bacteria convert nitrate (NO 3 ‐ ) to dinitrogen (N 2 ) gas through an anaerobic respiratory process in which the potent greenhouse gas nitrous oxide (N 2 O) is a free intermediate. These bacteria can be grouped into classes that synthesize a nitrite (NO 2 ‐ ) reductase (Nir) that is solely dependent on haem‐iron as a cofactor (e.g. Paracoccus denitrificans ) or a Nir that is solely dependent on copper (Cu...
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