Marinobufagin (MBG), a cardiotonic steroid from Rhinella toads, exhibits potent antitumor activity. However, their involvement in the mammalian neurotoxicity remains unclear. Therefore, this study evaluated the systemic (neuro)toxicological action of MBG to understand its central effects, particularly those associated with seizure-inducing activity. Initially, physicochemical, pharmacokinetic, and drug-likeness profiles and mo...

Accurate detection of high-risk human papillomavirus (HPV) is central to cervical cancer screening. Current diagnostic strategies increasingly prioritize broad-spectrum assays to maximize genotype coverage. However, theoretical genotype breadth does not necessarily translate into effective sample-level detection when clinically dominant targets are present at low copy number. Here, we evaluated this analytical trade-off using...

Aim We investigate the distribution of dispersal modes — endozoochory (via animal ingestion), synzoochory (via animal seed accumulation) and anemochory (via wind)—and seed mass of woody plants across Brazilian ecosystems, testing whether their relative abundance in local plant communities could be predicted by climate, edaphic factors and dispersal agents (e.g., the main vectors moving seeds). We expected climate to be the mai...

The red macroalgae Palmaria palmata is rich in protein and bioactive compounds and has been identified as a promising dietary ingredient for aquafeeds, with the potential to partially replace selected plant-based feed components. A digestibility trial and a growth trial were conducted to evaluate the potential of untreated and pretreated P. palmata as feed ingredients for juvenile European seabass ( Dicentrarchus labrax ), wit...

Codium tomentosum , a marine green macroalga is also produced in integrated multi-trophic aquaculture (IMTA) systems; however, its use in aquafeeds remains unexplored. This study evaluated the potential of C. tomentosum as a feedstuff for European seabass ( Dicentrarchus labrax ) and, for the first time, applied a high-solid-loading alkaline autoclave pre-treatment to enhance its nutritional value. Macroalgal biomass was treat...

Purpose The European Medicines Agency (EMA) guideline Declaration of Storage Conditions in the Product Information of Medicinal Products (CPMP/QWP/609/96/Rev.2) was last revised in 2007. Since then, advances in stability science, increased climatic variability, and evolving regulatory expectations have highlighted limitations in the framework, particularly the use of vague descriptors such as “room temperature” or “no special...

An experiment was conducted to evaluate the effects of broiler breeder age on the growth performance, nutrient metabolism, and intestinal histomorphometry of the progeny during early life. A total of 180 one-day-old Cobb 500 ® chicks, with an average initial body weight of 45.82 ± 2.21 g, were assigned to a completely randomized design with three treatments, six replicates, and 10 birds per replicate. The treatments consisted...

This study assessed the effect of dietary oil sources on tambaqui ( Colossoma macropomum ) exposed to acute cold stress. Three different oil sources were incorporated individually at 7% into the diets: fish oil (FO), a blend of linseed and palm oils (LO), or corn oil (CO). These diets differed in their n-6/n-3 polyunsaturated fatty acid (PUFA) ratios, with n-3 PUFA derived from EPA + DHA (FO) or linolenic acid (LO) and n-6 PUF...