I. Aoki results 29
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A hydrographic section along 55° E, south of 30° S, was visited from December 2019 to January 2020 as the first occupation under the Global Ocean Ship-based Hydrographic Investigations Program. The water column was measured from the sea surface to 10 dbar above the bottom with eddy-resolving station spacings and state-of-the-art accuracy. The upper profile was characterised by a conspicuous front between 42.5 and 43° S and a c...
Dense, cold waters formed on Antarctic continental shelves descend along the Antarctic continental margin, where they mix with other Southern Ocean waters to form Antarctic Bottom Water (AABW). AABW then spreads into the deepest parts of all major ocean basins, isolating heat and carbon from the atmosphere for centuries. Despite AABW’s key role in regulating Earth’s climate on long time scales and in recording Southern Ocean c...
The feeding ecology of two dominant lanternfishes Diaphus garmani and Diaphus chrysorhynchus was studied in the continental slope region of the East China Sea, off western Kyushu (31–33° N; 128–130° E). Stomach contents of D. garmani were composed mainly of crustacean zooplankton, such as copepods, euphausiids, decapod larvae and amphipods, and also of appendicularians. Stomach contents of D. chrysorhynchus were composed mainl...
Inhibition of hirame rhabdovirus growth by RNA aptamersNARA Subscribed
RNA aptamers are artificial nucleic acids that specifically bind to a wide variety of targets. They are an effective tool for pharmaceutical research and development of antiviral agents. Here, we describe four Hirame rhabdovirus ( HIRRV )‐ RNA aptamers ( H 1, H 2, H 3 and H 4) that we obtained from an in vitro process called the systematic evolution of ligands by exponential enrichment ( SELEX ). The HIRRV ‐ RNA aptamers speci...
Changes to the properties of Antarctic Bottom Water in the Australian-Antarctic Basin (AA-AABW) between the 1990s and 2000s are documented using data from the WOCE Hydrographic Program (WHP) and repeated hydrographic surveys. Strong cooling and freshening are observed on isopycnal layers denser than γn = 28.30 kg m−3. Changes in the average salinity and potential temperature below this isopycnal correspond to a basin-wide warm...
The minimum inhibitory concentrations (MICs) of 15 chemotherapeutic agents were tested against 146 Lactococcus garvieae strains isolated from 1999 to 2006 in Japan. The agents used included chloramphenicol, ciprofloxacin, erythromycin (EM), enoxacin, fleroxacin, florfenicol, kanamycin, lincomycin (LCM), norfloxacin, oxolinic acid, orbifloxacin, ofloxacin, benzylpenicillin, streptomycin and tetracycline (TC). Of the tested stra...
Cloning and characterization of Photobacterium damselae ssp. piscicida phospholipase: an enzyme that shows haemolytic activityNARA Subscribed
A phospholipase gene of Photobacterium damselae ssp. piscicida ( ppp ) was cloned from a genomic library and its nucleotide sequence was determined. The open reading frame consisted of 1218 bp encoding a protein of 405 amino acids with a predicted molecular mass of 46 kDa. The PPP had identities (53–55%) with phospholipase and haemolysin of Vibrio spp., while it showed low identities (23–26%) with glycerophospholipid cholester...
Feeding habits and gill raker morphology were examined for the three major planktivorous pelagic fishes, Japanese anchovy Engraulis japonicus , Pacific round herring Etrumeus teres and Japanese jack mackerel Trachurus japonicus , off the northern and western coasts of Kyushu, in the north‐eastern part of the East China Sea in the summer months of 2001. Using fishes in the same size range (80–140 mm, standard length), the stoma...
Putative virulence‐related genes in Vibrio anguillarum identified by random genome sequencingNARA Subscribed
The genome of Vibrio anguillarum strain H775‐3 was partially determined by a random sequencing procedure. A total of 2300 clones, 2100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence‐related genes...
Quinolone‐resistant strains of the fish‐pathogenic bacterium, Photobacterium damselae subsp. piscicida are distributed widely in cultured yellowtail, Seriola quinqueradiata (Temminck & Schlegel), in Japan. The quinolone resistance‐determining region (QRDR) was amplified with degenerate primers, followed by cassette ligation‐mediated PCR. Open reading frames encoding proteins of 875 and 755 amino acid residues were detected in...
Molecular cloning and functional analysis of Photobacterium damselae subsp. piscicida haem receptor geneNARA Subscribed
A haem receptor gene from Photobacterium damselae subsp. piscicida (formerly known as Pasteurella piscicida ) has been cloned, sequenced and analysed for its function. The gene, designated as pph , has an open reading frame consisting of 2154 bp, a predicted 718 amino acid residues and exists as a single copy. It is homologous with the haem receptors of Vibrio anguillarum hupA , V. cholerae hutA , V. mimicus mhuA and V. vulnif...
Long‐term changes in the assemblage of demersal fishes and invertebrates in relation to environmental variations in Tokyo Bay, JapanNARA Subscribed
Long‐term changes in the demersal assemblage, including both fishes and invertebrates, in Tokyo Bay, Japan, were examined between 1977 and 1995. A total of 255 species were collected, of which 113 were regarded as representative in terms of occurrence. Multivariate analyses of the representative species showed that fluctuations in the catch were related to three periods that reflected changes in relative abundance and species...
Reproductive ecology of Japanese anchovy off the Pacific coast of eastern Honshu, JapanNARA Subscribed
Spawning frequency of offshore migrant populations of Japanese anchovy Engraulis japonicus ranged from 0·34 to 0·90, and was higher than that of inshore Japanese anchovy. In addition, spawning frequency of offshore populations varied in response to the sex ratio. They spawned at water temperatures of 5.0, 8.6 and 12.6° C, indicating that the minimum critical water temperature of offshore Japanese anchovy was considerably lower...