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Application of Fibronectin-Coated Microparticles for Isolation of Human iPSC-Derived MSCsNARA Subscribed
BACKGROUND : Bone marrow–derived MSCs (BMMSCs) are limited by availability, donor variability, and age-related decline, highlighting the need for alternative MSC sources. Induced pluripotent stem cells (iPSCs) offer a scalable solution. This study introduces a novel three-dimensional culture platform based on fibronectin (FN)-coated microparticles to efficiently derive and expand human iPSC-derived MSCs (iMSCs). METHODS : The...
Reliable identification of seafood species is critical for fisheries management and product authentication, especially when morphological characteristics are lost during processing. In this study, a multiplex PCR system was developed to distinguish seven cuttlefish species (six Sepia spp. and Sepiella inermis) commercially distributed in the Korean seafood market. Species identity was first confirmed by amplifying a mitochondr...
Development and validation of a portable TDLAS system for gas chromatography–level quantification of methane emissions from riceNARA Subscribed
Rice cultivation is a significant source of agricultural methane (CH₄), yet routine quantification still relies heavily on gas chromatography (GC), which limits throughput and field deployment. Here, we evaluated a portable tunable-diode-laser absorption spectroscopy (TDLAS) detector (PMD) as an alternative to GC for measuring CH₄ released from pot-grown rice plants under field conditions. Weekly closed-chamber samples from fi...
The mineralization of organic matter at the sediment plays a crucial role in ecosystem functioning by facilitating the biogeochemical cycling of carbon and nutrients. This process not only supports nutrient availability for primary production but also regulates the long-term storage of carbon within sediments. To understand the biogeochemical processes associated with organic matter mineralization and nutrient regeneration, we...