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Molecular Ecology · 2018 · Vol. 27 · Issue 23 · Wiley
Sodiomyces alkalinus is one of the very few alkalophilic fungi, adapted to grow optimally at high pH . It is widely distributed at the plant‐deprived edges of extremely alkaline lakes and locally abundant. We sequenced the genome of S. alkalinus and reconstructed evolution of catabolic enzymes, using a phylogenomic comparison. We found that the genome of S. alkalinus is larger, but its predicted proteome is smaller and heavily...
Environmental Microbiology · 2015 · Vol. 17 · Issue 8 · Wiley
Summary The protein product of S accharomyces cerevisiae DFG 5 gene is a glycosylphosphatidylinositol ( GPI )‐anchored plasma membrane protein and a putative glycosidase/glycosyltransferase that links other GPI ‐anchored proteins to β‐glucans in the cell wall. Upon exposure to heat (41°C), DFG 5 deletion mutant dfg5 Δ displayed significantly enhanced heat tolerance as well as lowered level of reactive oxygen species and decrea...
Environmental Microbiology · 2015 · Vol. 17 · Issue 5 · Wiley
Summary The catabolic fate of the major monomeric sugar of red macroalgae, 3,6‐anhydro‐ L ‐galactose ( AHG ), is completely unknown in any organisms. AHG is not catabolized by ordinary fermentative microorganisms, and it hampers the utilization of red macroalgae as renewable biomass for biofuel and chemical production. In this study, metabolite and transcriptomic analyses of V ibrio sp., a marine bacterium capable of cataboliz...
Environmental Microbiology · 2015 · Vol. 17 · Issue 3 · Wiley
Summary Screening a library of overexpressing mutant alleles of the TATA ‐binding gene SPT15 yielded two S accharomyces cerevisiae strains ( MRRC 3252 and 3253) with enhanced tolerance to acetic acid. They were also tolerant to propionic acid and hydrogen peroxide. Transcriptome profile analysis identified 58 upregulated genes and 106 downregulated genes in MRRC 3252. Stress‐ and protein synthesis‐related transcription factors...