Lipid droplets (LDs) are ubiquitous subcellular organelles playing crucial roles in lipid and energy homeostasis. They are constantly generated in the intramembrane space of the endoplasmic reticulum (ER) through unique mechanisms. Upon maturation, they bud off from the ER outer membrane into the cytosol, travel through the cytosolic microtubular network, and make contacts to most of the other subcellular organelles to perform...