Koi herpesvirus ( KHV ) causes KHV disease ( KHVD ). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti‐ KHV antibodies have not yet been fully validated for KHV . Therefore, it was developed and validated an enzyme‐linked immunosorbent assay ( ELISA ) to detect...

Cyprinid herpesvirus 3 (Cy HV ‐3) is the aetiological agent of a serious infective, notifiable disease affecting common carp and varieties. In survivors, infection is generally characterized by a subclinical latency phase with restricted viral replication. The Cy HV ‐3 genome is difficult to detect in such carrier fish that represent a potential source of dissemination if viral reactivation occurs. In this study, the analytica...

Fish samples initially collected by local veterinarians on the common and koi carp farms in Poland between 2013 and 2015 as part of a KHV surveillance programme, when the water temperature was between 16 and 26 °C, and were also tested for CEV by qPCR . A partial 478 nucleotide fragment of the 4a gene was subsequently generated from 17 qPCR ‐positive common carp Cyprinus carpio samples from 36 farm sites tested during the peri...

A rhabdovirus was isolated in cell culture inoculated with tissue material from diseased grayling, T hymallus thymallus (L.), originating from a fish farm affected by a mortality episode in P oland. Diagnostics tests showed that the virus was not related to novirhabdoviruses known in E urope, nor to vesiculovirus‐like species, except p erch rhabdovirus ( PR hV) with which it shared moderate serological relations. However, RT ‐...

Viral haemorrhagic septicaemia ( VHS ), caused by the novirhabdovirus viral haemorrhagic septicaemia virus ( VHSV ), causes significant economic problems to European rainbow trout, O ncorhynchus mykiss ( W albaum), production. The virus isolates can be divided into four distinct genotypes with additional subgroups. The main source of outbreaks in European rainbow trout farming is sublineage Ia isolates. Recently, this group of...