Summary A total of 1940 isolates from gut samples of 60 bumblebees representing Bombus pascuorum , Bombus terrestris, Bombus lucorum and Bombus lapidarius was collected and identified through state‐of the‐art taxonomic methods. The bacterial species diversity in these Bombus species exceeded that suggested by phylotype analysis through 16S rRNA amplicon sequencing, and revealed that B. pascuorum and B. terrestris had a unique...