Decapod iridescent virus 1 (DIV1) causes severe disease outbreaks in shrimp and crab culture areas. A simple, rapid, and visual DIV1 assay is important for the control of viral diseases. This study presented a novel DIV1 detection method that combines recombinase polymerase amplification (RPA) and lateral flow strip (LFS). After selecting primers and probes, we optimised the concentration of the reverse primers, reaction time,...