Stable isotope analysis is used often in ecological studies to examine dietary patterns of consumers and food web structure, yet we still know little regarding some underlying assumptions, including for well-studied species like rainbow trout Oncorhynchus mykiss . We assessed δ 13 C and δ 15 N discrimination (∆) and turnover rates in blood, liver and white muscle of rainbow trout fed natural prey items (sculpin and chironomids...

Assimilation efficiency is a critical assumption of stable isotope mixing models and bioenergetics models, yet few studies examine how assimilation efficiency influences modeling inferences. We conducted laboratory experiments to determine rainbow trout ( Oncorhynchus mykiss ) assimilation efficiencies. Assimilation efficiency averaged 55.8% (SE ± 0.90) and 64.5% (SE ± 1.98) at the 10% and 25% ration levels, respectively, and...