Invasive species adversely affect biodiversity, but little is known about how ecologically similar invaders interact. Such intraguild interactions may drive non‐native community dynamics and their broader impacts on biodiversity. In native systems, intraguild interactions are widespread strong eco‐evolutionary pressures; understanding whether similar patterns occur among invaders is key to predicting invasion outcomes. We inve...

The success of non‐native species depends on their ability to find food, which may ultimately lead to competition with native species and contribute to biodiversity loss in invaded ecosystems. Understanding which food resources are consumed is therefore crucial for evaluating how non‐native species mechanistically fit into native biological communities. Non‐native species may be predators or competitors of native species or ma...

In their article, Littleford‐Colquhoun et al. (2022) advise against using arbitrary relative read abundance (RRA) thresholds (i.e., minimum sequence copy thresholds) for removing low‐abundance sequences since they can increase false negative rates in dietary DNA metabarcoding data sets. The main criticisms presented against these widespread methods are that they (i) are arbitrary, often existing as standard values or defined b...

Dietary analysis using DNA metabarcoding is a powerful tool that is increasingly being used to further our knowledge of trophic interactions in highly complex food webs but is not without limitations. Omnivores, the most generalist of consumers, pose unique challenges when using such methods. Here, we provide the rationale to understand the problems associated with analysing the complex diets of omnivores. By reviewing existin...