Tobacco ( Nicotiana tabacum L.) is an economically important crop, widely cultivated in China. Since 2021, a new leaf spot disease has been seen frequently in Enshi, Xiangyang, Yichang, and Shiyan, all in Hubei Province, China. Diseased leaves exhibiting roundish lesions with greenish-yellow edges were collected to determine the causal agent in summer 2022. After isolation, morphological characterization, and molecular identif...