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Optimising the method to develop spheroids from MDA-MB-468 human triple negative breast cancer cellsNARA Subscribed
Purpose Three-dimensional (3D) spheroid models are increasingly used to emulate the tumour microenvironment for preclinical drug screening. This study aimed to optimise and assess spheroid formation from MDA-MB-468 triple-negative breast cancer (TNBC) cells using hanging drop, liquid overlay, and rigid scaffold methods under normal oxygen (NOC) and low oxygen (LOC) culture conditions. Methods Spheroids were generated and chara...