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Evaluation of Three Viral Capsid Integrity qPCR Methods for Wastewater-Based Viral SurveillanceNARA Subscribed
Capsid Integrity qPCR (CI-qPCR) assays offer a promising alternative to cell culture-based infectivity assays for assessing pathogenic human virus viability in wastewater. This study compared three CI-qPCR methods: two novel (Crosslinker, TruTiter) and one established (PMAxx dye). These methods were evaluated on heat-inactivated and non-heat-inactivated ‘live’ viruses spiked into phosphate-buffered saline ( PBS) and wastewater...
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